3/17/2023 0 Comments Spore worksThe mobile phase was 0.1% ( v/v) formic acid (A) and methanol (B). A C18 Hypersil Gold column (3 µm, 2.1 × 100 mm, Thermo Scientific) was utilized for separation at 35 ☌ with a flow rate of 0.3 mL/min. The Thermo Dionex Ultimate 3000 UHPLC system was used for chromatographic analysis. Herein, we report the spatial distribution of bibenzyls, analysis of gene expression patterns, evaluation of antioxidant potentials, adaptation to UV-B radiation, and spore germination rate in a gender and region-specific pattern. polymorpha increased after UV-B radiation meanwhile, bibenzyls were proven to be able to accelerate spore germination, suggesting the bibenzyls play an important role in spore propagative protection and stress adaptation as antioxidants. In addition, the expression level of MpSTCS1A and the content of total phenolic acid in the thallus of M. The expression patterns of two key genes in the bibenzyl biosynthesis and the antioxidant capacity of the above four parts exhibited the same trend. polymorpha (ArM and TFM, respectively), rather than the antheridiophore and thallus of male M. Supported by high mass accuracy and in situ tandem MS (MS/MS), various bibenzyls were identified as predominate constituents, which were further observed in the archegoniophore and thallus of female M. polymorpha at the reproductive stage has been visualized for the first time. In this study, with the aid of one direct and sensitive ionization method: airflow-assisted desorption electrospray ionization (AFADESI) MSI, the spatial distribution of metabolites in M. These works broaden our understanding of the significance of bibenzyls in spore propagation and environmental adaptation.Īmong the modern techniques employed for plant metabolomics, mass spectrometry imaging (MSI) has developed as a powerful approach for microscopically visualizing the molecular distributions of plant tissue, in an untargeted and label-free way. Moreover, lunularic acid and extract of archegoniophore at a certain concentration can stimulate the spore germination under normal conditions and UV-B stress. The expression level of MpSTCS1A, and the content of total phenolic acid was increased after UV-B irradiation, suggesting bibenzyls play an important role in UV-B tolerance. In line with the spatial feature of bibenzyls, higher MpSTCS1A and Mp4CL expression levels and antioxidant ability were exhibited in the archegoniophore. The region-specific gene expression and antioxidant activities were characterized. polymorph a, where lunularic acid was found to focus in the central region and bisbibenzyls were enriched in the periphery. Bibenzyls were found to largely exist in the female receptacle of M. In this study, spatial distributions of the bibenzyls within Marchantia polymorpha L., the model species of liverworts, were mapped using airflow-assisted desorption electrospray ionization imaging mass spectrometry. As characteristic constituents of liverworts, the bibenzyls are efficient antioxidants. Liverworts, considered to be the first plant type to successfully make the transition from water to land, can resist different oxidative stress.
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